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Fig. 4. XGrhl1 is downstream of the BMP4 receptor, and can modulate
endogenous BMP4-responsive targets. (A) Dissociated animal cap assays were
performed as indicated in the schematic. Dispersed animal pole cells were
incubated in increasing doses of recombinant human BMP4 (hrBMP4) (B,C) or
one-cell embryos were injected with XGrhl1 mRNA (D), allowed to
develop to stage 9, and animal pole explants were dissected, dispersed and
allowed to re-aggregate. Aggregates were allowed to mature until stage 18,
harvested, RNA prepared and assayed by semi-quantitative RT-PCR. (B) Exposure
of dissociated ectodermal cells to hrBMP4 results in an increase in
epidermal-specific gene expression, including XGrhl1 and
XK81A1. (C) XGrhl1 is not an immediate early response gene.
Dissociated caps were incubated in BMP4 in the presence/absence of the protein
synthesis inhibitor cycloheximide (10 µg/ml; CHX). (D) Ectopic expression
of XGrhl1 in dispersed cap cells results in upregulation of
epidermal-specific gene expression.
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