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Fig. 2. sox9bb971 linkage map and sox9b expression, and sox9b morpholino-treated embryos. (A) LG3 (distance in cM), with red markers shown to be deleted, blue markers shown not to be deleted, and black markers untested. Region deleted, pale red. (B,C) Lateral views of 24 hpf embryos probed for sox9b expression. (B) wild type; (C) sox9bb971. Mutant embryos lack sox9b expression. (D,E) Dorsal, right side view of cranial crest in four-somite stage embryos probed for sox9b expression. (D) Wild type; (E) embryo injected with sox9b splice-directed MO showing retention of sox9b transcript in the nuclei. Scale bars: in C, 100 µm for B,C; in E, 100 µm for D,E.





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