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Fig. 2. Plasma and S1P rescue early morphogenesis of the dorsal pancreas in
Cdh2-/- explants. Pancreatic-gut explants from 9.5 dpc
wild-type (WT; A,G) and Cdh2-/- (B-F) embryos stained with
Pdx1 antibodies. (B-F) Explants from 9.5 dpc Cdh2-/-
embryos incubated in the presence of beads soaked in PBS (negative control;
C), plasma from 15.5 dpc wild-type embryos (D), 0.1 µM S1P (E), 0.2
µg/ml PTX+0.1 µM S1P (PTX beads were pre-grafted 2 hours before S1P
beads were added) (F), and explants from 9.5 dpc wild-type embryos incubated
with (G) or without (A) beads soaked in 0.2 µg/ml PTX. The beads were
grafted on the dorsal side of the gut, at the site of the presumptive dorsal
pancreas. After 48 hours in culture, the explants were stained with Pdx1 and
photographed. Vb, ventral pancreatic bud; db, dorsal pancreatic bud; s,
stomach; i, intestine; gb, gall bladder; pla, plasma. Scale bar in A: 100
µm.
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