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Fig. 4. Secretory cells express the Notch ligand Delta and their numbers are
increased in aeiAR33, a mutant lacking DeltaD. (A)
Intestine of adult mouse heterozygous for a lacZ knock-in at the
Delta1 (Dll1) locus. ß-Galactosidase activity (blue) is
a reporter for present or past expression of Delta1 expression; the
stain is seen in scattered cells, many of which can be clearly identified as
goblet cells. (B) Expression of Notch pathway components in zebrafish gut,
analysed by RT-PCR. deltaC and deltaD are strongly expressed
in the gut, while deltaA and deltaB are not. (C) DeltaD
(zdd-2 immunostain in green, arrows) is visible in a subset of secretory cells
(2F11, red) in the wild-type zebrafish intestine at 4 days. (D,E) Sections of
intestinal bulb of 5-day-old wild-type larvae (D) and
aeiAR33 homozygotes (E) stained with 2F11; TOPRO-3 nuclear
stain is red. (F) The proportion of 2F11-positive cells is increased all along
the length of the gut in aeiAR33 when compared with wild
type; data points show mean and s.e.m. from counts of sections of 12 larvae of
each genotype. (G) Total numbers of epithelial cells per section for the same
set of specimens.
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