spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Intestinal phenotype of mibta52b homozygotes. (A,B) 2F11 staining in the intestinal bulb, comparing three representative pairs of wild-type (A) and mibta52b (B) specimens at 3 days. The vast majority of epithelial cells are switched to a secretory fate in mibta52b. (C,D) At the same time, the number of cells expressing DeltaD (zdd-2 antibody, green, indicated by arrows in C) is greatly increased in the mutant. Membrane localization of DeltaD in mibta52b reflects the failure of Mib-dependent Delta internalization. (E,F) 4E8 staining (green) shows deficit of absorptive cells in three-day mibta52b. (G,H) 2H9 staining (green) shows abnormal epithelial cell polarity in mibta52b. (I,J) Staining of the basement membrane with 5F11 (green) is also fainter in mibta52b than in wild-type. (K,L) At 4 days, many dying cells are seen in mibta52b (L) but not in wild type (K). (A-L) TOPRO-3 nuclear stain is red.





Right arrow Return to article