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Fig. 6. Epiblast cells require hab to exhibit cell shape changes in a
cell-autonomous manner. Optical sections through the exterior (A) and interior
(B) layers of the epiblast of live wild-type and homozygous mutant
hab embryos at 80% epiboly. Average aspect ratio (length/width) and
surface area of wild-type and mutant cells were compiled from the outlines of
cells (20-25 cells/embryo) for each layer in a total of three wild-type and
four mutant embryos. Cell-autonomous effect of hab on cell shape in
the exterior (C) and interior (D) layers of the epiblast.
Wild-type or mutant cells were transplanted into the epiblast of a wild-type
host and analyzed at the 80% epiboly stage. Transplanted cells are brown due
to a fixable tracer. Data was compiled from outlines of donor cells (20-25
cells/host embryo) in a total of 15 chimeras, eight with cells from a
wild-type donor and seven with cells from homozygous mutant hab
donors. Note that aspect ratios and surface area of transplanted cells are
almost identical to their live wild-type and mutant counterparts. Panels are
orientated with the animal pole (AP) to the top and dorsal (D) to the
right.
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