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Fig. 5. Presence of stem/progenitor cells in cultures of E12 blood. (A) Osteogenic cell differentiation after 10 days of treatment in osteogenic permissive medium. Colonies stained positive for ALP. (B) Adipogenic differentiation after stimulation with adipogenic-inductive medium for 10 days; adipocytes are shown. (C) Chondrogenic differentiation after 21 days in chondrogenic medium. E12 blood cells formed a small cartilage-like pellet, in which the extracellular matrix was rich in proteoglycans, as detected by Toluidine Blue staining.





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