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Fig. 1. (A) Structure of the 5' regulatory region of mouse Nfatc1, which contains two independent promoters, P1 and P2, for transcription of Nfatc1.{alpha} and Nfatc1.ß isoforms, respectively. (B) RT-PCR analysis using exon (isoform)-specific primers showing that the Nfatc1.{alpha} isoform is abundant in the cultured primary E11.5 endocardial cells (ECC) and its transcripts is detected by a 35-cycle amplification, whereas the Nfatc1.ß isoform is detected only after a 40-cycle amplification. The structures of the isoforms are shown on the left.





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