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Fig. 7. Autoregulation of Nfatc1 enhancer activity. The BB-HSP-lacZ reporter transgenic line was crossed into the existing Nfatc1-null mutant line. Compared to the heterozygous littermates (A-C), inactivation of Nfatc1 greatly reduced the pro-valve endocardial enhancer activity of the BB-HSP-lacZ reporter construct (D-F). A consistent reduction of endocardial lacZ expression is shown in the OFT (arrow) and AVC (arrowhead) of the E11.5 heart when crossed into Nfatc1-null background





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