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Fig. 1. Mammary epithelial expression of Myc during pregnancy affects pup survival
postpartum. (A) Oligonucleotide microarray of analysis of endogenous
Myc expression during mouse mammary gland development. Total RNA (30
µg) was isolated from the mammary glands of three separate FVB wild-type
mice and hybridized to Affymetrix Mu6500 GeneChips as previously described
(Master et al., 2002). Raw
expression data are indicated on the y-axis. Black boxes indicate
detectable expression, whereas gray boxes indicate samples in which
Myc expression was not detected. Gene expression changes determined
to be statistically significant by the analysis described in Master et al.
(Master et al., 2005) are
indicated by vertical arrowheads. x indicates gene expression changes
determined not to be statistically significant. Fold-changes in Myc
expression, as compared with the baseline (`B') expression of 10-week-old G0P0
animals, are indicated at the top of each column. (B) Effect of MYC
transgene expression during pregnancy on postpartum pup survival. MTB/TOM mice
were administered 2.0 mg/ml doxycycline in their drinking water to induce
MYC transgene expression during the time period of pregnancy
indicated at the top. Parturition occurred after 18.5 days of gestation, and
pup survival within the first 24 hours was assessed. Shaded bars indicate
induction conditions for which all pups died within the first 24 hours of
parturition. Non-shaded bars indicate induction conditions in which all pups
survived beyond 24 hours of parturition. Hatched bars indicate induction
conditions in which a fraction of the litter died within 24 hours of
parturition. The effects of induction conditions on pup growth are indicated
as follows: +, affected; -, not affected; N/A, not applicable because of pup
death. The light-gray region from D12.5-D15.5 of pregnancy indicates the
window of highest susceptibility to MYC induction.
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