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Fig. 4. Mammary epithelial expression of MYC from D12.5-D15.5 of pregnancy promotes
precocious expression of markers of lactation. (A) Northern hybridization
analysis of total RNA extracted from MTB and MTB/TOM mouse mammary glands
induced with doxycycline from D12.5-D15.5 of pregnancy and harvested at 1-day
increments from D12.5 pregnancy through D1PP. Northern blots were hybridized
with probes specific to the human MYC transgene (MYC Tg),
endogenous mouse Myc, Csnd and prolactin-inducible protein
(Pip). Ethidium bromide staining of 28S RNA is shown as a loading
control. (B) Western blot analysis of milk protein expression. Mammary protein
lysates from uninduced MTB and MTB/TOM mice at D12.5 pregnancy and MTB and
MTB/TOM mice induced from D12.5-D15.5 of pregnancy were separated by SDS-PAGE
and transferred to nitrocellulose. Mammary lysates from FVB wild-type virgin
mice and mice at D18.5 of pregnancy are included as controls. Membranes were
probed with polyclonal antiserum specific to mouse milk proteins and
ß-tubulin. Expression of 
-casein and ß-casein are
indicated.
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