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Fig. 5. MYC expression during pregnancy induces abnormal mammary epithelial
proliferation. (A) BrdU immunohistochemistry of uninduced MTB and MTB/TOM mice
at D12.5 of pregnancy; MTB and MTB/TOM mice induced with doxycycline from
D12.5-D13.5 sacrificed at D13.5 of pregnancy; and MTB and MTB/TOM mice induced
from D12.5-D15.5 and sacrificed at D16.5 of pregnancy. Mice were injected i.p.
with 50 mg/kg BrdU 2 hours prior to sacrifice. Histological sections from
number four mammary glands were assessed for incorporation of BrdU by IHC
analysis. Results are representative of three animals per group. (B) Northern
hybridization analysis of total RNA extracted from the mammary glands of MTB
and MTB/TOM mice induced with doxycycline as indicated. Total RNA was
separated by gel electrophoresis, transferred to nitrocellulose and hybridized
with a radiolabeled probe specific to cyclin A mRNA. Ethidium bromide staining
of 28S RNA is shown as a loading control.
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