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Fig. 6. Mammary epithelial expression of MYC from D12.5-D15.5 of pregnancy promotes
premature Stat5 hyperactivation. MTB and MTB/TOM mice induced with doxycycline
from D12.5-D15.5 were sacrificed at 24-hour intervals from D12.5
pregnancy-D1PP. Mammary protein lysates were separated by SDS-PAGE and blotted
onto nitrocellulose membranes. Blots were probed with antibodies specific to
Y694/Y699 phosphorylated Stat5a/b, total Stat5a/b, and ß-tubulin. Results
are representative of experiments performed on two separate sets of
animals.
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