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Fig. 8. MYC expression promotes activation of the Stat3 and Tgfb3 involution
pathways. (A) The Y705-phosphorylation status of Stat3 was determined by
western blot analysis. Mammary protein lysates from MTB and MTB/TOM mice
induced with doxycycline from D12.5-D15.5 of pregnancy and sacrificed at
24-hour intervals from D12.5 of pregnancy through D1PP were separated by
SDS-PAGE and transferred to nitrocellulose membranes. Lysates from FVB
wild-type mice harvested during virgin development, D18.5 of pregnancy and D2
of regression were included as controls. Membranes were probed with antibodies
specific to Y705-phosphorylated Stat3 and total Stat3. Results are
representative of experiments performed on two separate sets of animals. (B)
Total RNA was extracted from mammary glands of MTB and MTB/TOM animals induced
from D12.5-D15.5 of pregnancy and sacrificed at D17.5-D1PP. Expression of
Tgfb3 mRNA and a direct Tgfb3 target, Serpine1, were
assessed by northern hybridization analysis with radiolabeled probes.
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