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Fig. 2. GFP fluorescence of day 4 Mixl1GFP/w EBs.
Low-magnification bright-field (A) and epifluorescence (B) images showing that
most EBs contain GFP-positive cells. (C) Confocal image showing that
GFP-positive cells are present both on the surface and distributed throughout
the EB. (D) High magnification of the EB in C, showing GFP-positive cells
located on the surface of the EB have a flattened morphology reminiscent of
endoderm (arrowhead).