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Fig. 6. ULT1 and ULT2 mRNA expression patterns in seedlings and embryos. RNA localization by in situ hybridization with ULT1 (A,D,J,K,N,P) and ULT2 (B,E,G,L,M,O) antisense probes hybridized to wild-type Ler seedlings (A-C) and embryos (D-Q). (A-C) Longitudinal sections through 7-day-old seedlings. (A) ULT1 transcripts are localized throughout the vegetative SAM and in young leaf primordia. (B) ULT2 transcripts are not detected in seedlings. (C) Control hybridization with an ULT1 sense probe. (D-H) Longitudinal sections through mature embryos. (D) ULT1 expression is restricted to the embryonic SAM (arrowhead). (E) ULT2 transcripts can be detected in the embryonic SAM and RAM (arrowheads). (G) Higher magnification of ULT2 expression in the RAM. (F,H) Control hybridization with ULT1 and ULT2 sense probes, respectively. (I-Q) Longitudinal sections through embryos at different stages of embryogenesis. (I) Early globular stage. (J) Eight-cell-stage embryo. (K) Late triangle stage. (L) Early heart stage. (M) Late heart stage. (N) Early torpedo stage. (O) Torpedo stage. (P,Q) Bending cotyledon stage. (I,Q) Control hybridizations with an ULT1 sense probe. Scale bars: 50 µm in A-F; 25 µm in G-Q.





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