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Fig. 8. ULT1 and ULT2 T-DNA insertion alleles. (A) RT-PCR on wild type Ler, ult1-3 and ult2-1 T-DNA insertion mutant inflorescences. ULT1 transcripts could be amplified from Ler (wild-type) plants but not from ult1-3 plants, while ULT2 transcripts were detected in wild-type Col-0 and ult2-1/+ heterozygous plants but not in ult2-1 homozygous plants after 40 cycles of PCR. However, after 45 cycles a faint signal corresponding to correctly spliced ULT2 transcript was detected in the ult2-1 homozygous lane. EF1 was amplified as a control. Additional control amplification reactions were run with each set of primers using genomic DNA (gDNA) as a template. (B) Floral organ number in Ler, ult1-1, ult1-2 and ult1-3 mutant plants. Graph shows the mean number of organs in the first ten flowers of 10 plants (n=100 flowers), and the standard error is indicated. (C) Mean days to bolting after germination for Ler, ult1-1, ult1-2 and ult1-3 plants (n=10 plants). The standard error is indicated. (D) Floral organ number in ult1-3 homozygous plants, ult1-1/+ heterozygous plants and ult1-1/ult1-3 plants. Graph shows the mean number of organs in the first ten flowers of four or six plants (n=40 flowers for ult1-3 and n=60 flowers for the other genotypes), and the standard error is indicated. (E) Mean days to bolting after germination for ult1-3 homozygous plants, ult1-1/+ heterozygous plants and ult1-1/ult1-3 plants (n=4 plants for ult1-3 and n=6 flowers for the other genotypes). The standard error is indicated.

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