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Fig. 5. Defects in retinal growth in
Bmpr1a-/fx;Bmpr1b-/-;Cre
double mutant mice. (A) Side views of neonates. The double null mutant has an
anophthalmic phenotype (bottom). (B) Side views of the eyes of E12.5 embryos.
Reduced eye size and incomplete closure of the retinal pigmented epithelium
ventrally are seen in the double mutant (bottom). (C) Coronal sections of
embryos shown in B. (D) Cell-death analyses at E11.5 indicate a significant
increase in TUNEL-positive cells (green) in the mutant retina (counterstained
with propidium iodide). (E-G) Although there is no significant change in the
percentage of BrdU-positive cells up to E11.5 (green/yellow cells) (E,G),
there is a rapid reduction of cell proliferation by E12.5 (F,G; broken line in
F outlines the degenerating mutant retina; a relatively large error bar in the
E11.5-12.5 mutant group reflects a spectrum of phenotypic severity during
these stages). le, lens; nr, neuroretina. Scale bar: 500 µm in B; 200 µm
in C; 100 µm in D-F.
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