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Fig. 6. Bmpr1a^-/fx;Bmpr1b^-/-;Cre double null mutants display a range of defects in the expression of retinal marker genes. Analyses of gene and protein expression in coronal sections of embryonic retina at the indicated stages by radioactive in situ hybridization (A,C,D), DIG in situ hybridization (E,F,G) or immunostaining (B,H). (A) At E11.5, expression of Chx10 is attenuated in the mutant retina (right) compared with control. (B) CyclinD1 is specifically absent in the mutant retina (right), whereas protein expression in the adjacent lens tissue is maintained. (C,D) Lhx2 and Rx, two early pan-retinal markers, are expressed in the mutant retina at comparable levels with the control, despite the apparent degenerative phenotypes at this stage. (E) The mutant retina fails to initiate retinal neurogenesis at E11.5 as indicated by the loss of Math5 expression. Arrows indicate comparable Math5 expression both in the diencephalons of both control and mutant. This is not simply a developmental delay, as Math5 is absent even at E12.5 (not shown). (F) Brn3b, a downstream target of Math5 expressed in the newly born ganglion cells in the central retina, fails to be induced in the mutants. (G) Expression of Pax6 is maintained in the mutant. (H) Neuronal tubulin expression is detected in the mutant retina. le, lens; nr, neuroretina; rpe, retinal pigment epithelium. Scale bar: 100 µm.

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