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Fig. 4. Primitive streak graft using transgenic chick tissue. Wild-type chick host embryo at HH stage 3d grafted with a segment of the primitive streak obtained just caudal to Hensen's node from a transgenic chick donor embryo. (A) The location of the grafted tissue in the host (white arrowheads) is shown immediately after grafting. After 1 hour the graft had fully integrated. (B) The grafted embryo after 20 hours of incubation in EC culture. (C) Following immunocytochemistry with anti-GFP antibody and DAB, the grafted cells can be identified within the heart tube and as bilateral streams extending caudally into the area pellucida. The horizontal line indicates the level of the section shown in D. (D) Paraffin sections of the embryo (12 µm) show that the labeled cells (brown) contributed mainly to the heart mesoderm at the level (anterior intestinal portal) of the fusing lateral body folds. Scale bars: in A,D, 150 µm; in B, 250 µm for B,C.





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