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Fig. 5. Somite graft using transgenic chick tissue. (A) Three somites from a
transgenic embryo (black arrowheads) were grafted into the left side of a
48-hour wild-type chick host embryo (ventral side up in EC culture). (B) After
a 24-hour incubation, the integrated graft was observed on the left side of
the whole-mount embryo (dorsal view) after processing for immunocytochemistry
with an anti-GFP antibody and DAB staining. (C) Higher magnification view of
the integrated graft in B. (D) Section at the level indicated by the
horizontal line in B. Paraffin section (12 µm) at the level of the donor
somitic tissue demonstrates that cells integrate into the host embryo in the
neural tube, dermomyotome and heart. Scale bars: in B, 200 µm for A,B; in
C, 200 µm for C,D.
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