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Fig. 4. Decreased expression of the E-cadherin reporter gene after deletion of
intron 2 sequences at later embryonic stages. (A-C,F-H) Ecad-In2flox (A-C) and
Ecad-In2floxdel embryos (F-H) were stained for ß-gal expression, at the
indicated developmental stages, for 45 minutes (45', C,H) or overnight
(ON, A,B,F,G). Increased expression in the skin is observed in Ecad-In2flox
embryos during development. The E-cadherin locus without intron 2 is
activated, but expression levels are significantly lower. (D,E,I,J) Isolated
organs of E16.5 Ecad-In2flox (D,E) and Ecad-In2floxdel embryos (I,J) stained
for ß-gal expression for 45 minutes (D,I) or overnight (E,J). High
expression levels are found in the pancreas, stomach, gut and thymus of
Ecad-In2flox embryos (D). After 45 minutes of staining, expression in organs
of Ecad-In2floxdel embryos is only detected in pancreas and esophagus (I).
After overnight incubation, lung epithelium is only weakly stained (J). (K-X)
High magnification of sagittal sections of E11.5 embryos with the Ecad-In2flox
(K-Q) and Ecad-In2floxdel allele (R-X). Organs or regions of the embryo are
labeled in each figure. After sectioning, E-cadherin-specific expression can
be observed in all tissues in Ecad-In2flox embryos, but no expression is found
after deletion of intron 2, except for a faint expression detected in the
pancreas primordium (V). Scale bars: 1 mm in A-J; 100 µm in K-X.
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