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Fig. 6. Cells in the lens and salivary glands absolutely require cis-regulatory
elements in intron 2 for activation of ß-gal transcription. (A-F) X-gal
staining in lenses of Ecad-In2flox embryos shows high intensities (A,C,E),
whereas no expression is found in lenses of Ecad-In2floxdel embryos (B,D,F).
Lenses at stages E10.5 (A,B), E12.5 (C,D) and E14.5 (E,F) are shown. (G-P)
Analysis of E-cadherin-specific expression in mandibular salivary (arrow) and
thyroid (white arrowhead) glands at E16.5 (black arrowhead marks meninges).
Heads of Ecad-In2flox (G) and Ecad-In2floxdel embryos (H) were cut prior to
X-gal staining and viewed from bottom. In Ecad-In2flox high expression is
found in the salivary and thyroid glands (G), but no staining is observed in
salivary glands of Ecad-In2floxdel embryos (H). (I-P) Transverse sections of
Ecad-In2flox (I-L) and Ecad-In2floxdel heads at E16.5 (M-P). Sections of
salivary (I,M) and thyroid glands (J,N), trachea (K,O) and meninges (L,P).
Scale bars: 100 µm in A,B,J,N; 250 µm in C,D; 500 µm in E,F; 1 mm in
G,H; 50 µm in I,K,L,M,O,P.
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