spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. Intron 2 sequences are required for maintaining E-cadherin expression. (A-D) Whole-mount ß-gal staining of F1 embryos of CK14-Cre males crossed to Ecad-In2flox females. A slightly reduced staining is seen at E12.5 (A) in embryos where Cre was active (+/{Delta}) compared with control embryos with no Cre allele (+/flox). Further reduction is found in E13.5 (B), E14.5 (C) and E16.5 (D) embryos. Tissues where Cre was not active (lens, gut loops) are still strongly stained. (E,F) Whole-mount ß-gal staining of F1 embryos of CK19-Cre males crossed to Ecad-In2flox females. No difference in gene activity is observed at E9.5 (E), but decreased gene activity after intron 2 deletion is visible in E10.5 embryos (F). (G-J) Transverse sections of the gut tube (G,H) and sagittal sections of the pharynx (I,J) of whole-mount stained E10.5 control (+/flox, G,I) and Ecad-In2flox/CK19-Cre embryos (+/{Delta}, H,J). Scale bars: 500 µm in E; 1 mm in A,B,F; 2 mm in C,D; 50 µm in G-J.





Right arrow Return to article