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Fig. 5. Lgl2 promotes basolateral and inhibits apical identity. (A) Injection of 5
ng GFP did not affect the cells. (B,C) Injection of Xenopus
Lgl2 caused loss of pigment and also a block in cytokinesis at high doses
(B, 5 ng; C, 0.5 ng). (D-K) Injection of GFP (D-G) or Lgl2
(H-K) and immunostaining with the markers shown in each panel.
GFP-injected embryos were entirely normal. (I) Injection of
Lgl2 caused a reduction in keratin to the levels normally seen in the
basolateral region (arrow) and loss of tight junctions (cingulin, arrowhead).
(J,K) Injection of Lgl2 caused ectopic localisation of
ß1-integrin (J) and occludin (K) to the apical side (arrow) and loss of
tight junctions (arrowhead). (L) diagrammatic representation of phenotype,
colours as above. Experiments were carried out three times in both albino and
pigmented embryos (except for the keratin where the staining is obscured by
the pigment and therefore was carried out only in albinos), and the same
result was obtained in both.
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