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Fig. 6. Time-lapse images showing the gradual but direct depigmentation of the
apical side by Lgl2. A pigmented embryo was injected animally with
Lgl2 RNA at the two-cell stage and filmed. A small site of
cytoplasmic leakage helps to verify the site of injection. Evidence of apical
membrane disruption starts as a concentration of pigment spots (arrow) that
appear quite suddenly and spread quickly. The even distribution of
pigmentation is lost and the pigment is gradually cleared from the apical
side. Interestingly, pigment becomes concentrated to the periphery of the
apical domain. There is no evidence of inner cells coming to the surface of
the embryo or outer cells falling in.
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