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Fig. 7. aPKC and Lgl act by a process of mutual inhibition. (A,B) GFP-Lgl was
injected on its own (A) or with aPKC (B). Addition of aPKC inhibited the
basolateral localisation of GFP-Lgl2. GFP was visualised by using an anti-GFP
antibody. (C,D) Overexpression of Lgl2 inhibited the apical localisation of
aPKC but overexpression of GFP did not. (E-G) Lgl2, but not
GFP injections, can rescue the apicalisation caused by injecting
aPKC. There are more rounded cells in aPKC plus
GFP-injected embryos than in aPKC plus
Lgl2-injected embryos. The graph shows the average percent of embryos
with apicalised cells from three experiments. The experiment was scored blind
as for Fig. 2.
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