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Fig. 3. The SoxB1 C-terminal region contains multiple sequence signals for turnover
in macromere lineages that are not required for degadation in micromere
progeny. Zygotes were injected with GFP-tagged (green box) SoxB1, from which
residues were deleted as indicated by the thinner black lines in the diagrams
of the constructs shown on the left. The DNA-binding domain (light blue box)
is bordered on each side by a nuclear localization signal motif (dark blue).
Embryos (at least 30) were examined for depletion of the protein in micromere
derivatives (primary mesenchyme cells) and macromere progeny (the vegetal
plate). All embryos treated with the same mRNA showed a consistent phenotype,
which is illustrated in the middle column. An asterisk marks the center of the
vegetal plate of each embryo. (A) Control, intact SoxB1-GFP. (B) SoxB1-GFP
from which almost the entire sequence 3' of the DNA-binding domain was
deleted was not destabilized preferentially in vegetal plate cells. (C-E)
Different portions of the C-terminal region are each sufficient to mediate
vegetal clearance. Scale bar: 20 µm.
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