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Fig. 7. Downregulation of SoxB1 mRNA in vegetal blastomeres requires
ß-catenin, but not Krl. Zygotes were injected with glycerol (control),
with mRNA encoding the indicated mRNAs, or with Krl-MO, allowed to develop to
the hatched blastula stage, and then assayed by whole-mount in situ
hybridization with a SoxB1 probe. (A) Blocking nuclearization of
ß-catenin by injection of C-cadherin mRNA blocks downregulation of
SoxB1 mRNA in vegetal blastomeres. (B) Neither knockdown of Krl
translation with a KrlMO nor its mis/overexpression by mRNA injection (Krl
MOE) detectably affects downregulation of SoxB1 message in vegetal
blastomeres. However, in the animal hemisphere, loss of Krl function leads to
a decrease in SoxB1 mRNA, whereas MOE up regulates it. This effect is
likely to reflect the operation of the SoxB1 negative-autoregulatory loop, as
discussed in the text and illustrated in
Fig. 5. Scale bar: 20
µm.
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