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Fig. 4. CycA and CycB are misregulated in twin. Anterior is leftwards in
all panels. The pair in each row was stained and photographed in parallel.
(A-D) CycA, red; fusome (1B1), green. (E,F) CycB, red; fusome, green. (A)
Wild-type (twin/+) chamber showing a cyst in region 1 expressing
CycA. Some of the CycA co-localized with the fusome, which appears yellow.
Posterior to this cyst are several highly branched fusomes. (B)
twinS1/Df germarium showing very strong CycA staining
throughout. CycA colocalizes with some fusomes even towards the posterior of
the germarium (yellow). Fusomes are present, and contain branches. (C)
Wild-type (twin/+) germarium showing CycA in two cysts in region 1.
One of these cysts colocalizes CycA to the fusome. (D)
twinry3/Df germarium showing CycA staining throughout. The
misexpression phenotype is not as prominent as in the strongest allele, S1.
The fusomes are clearly visible and highly branched. (E) Wild-type
(twin/+) germarium showing CycB in two cysts in region 1. Both of
these cysts show CycB co-localizing with the fusome. (F)
twinS1/Df showing very strong CycB staining throughout
germarium. The unstained regions are sites of dying cysts. CycB colocalizes
with the fusome in all the cysts. Staining with anti-CycE antisera did not
reveal a difference between twin/Df and twin/+ germaria (not
shown).
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