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Fig. 5. Cytoplasmic Bam is reduced in twin germaria. (A,B) BamC, red; germ cells (Vasa), green. (A',B') red channel alone. The BamC antiserum is specific to the cytoplasmic form of Bam (McKearin and Ohlstein, 1995). Bam can also localize to the fusome, but that fraction is not detected by the available antibodies. Vasa is expressed in germ cells. Anterior is leftwards in all panels. All samples were stained and photographed in parallel. (A,A') Wild-type (OR) germarium showing BamC staining in a cyst in Region 1. All the germ cells express Vasa. (B,B') twinS1/Df germarium showing very reduced BamC staining. twin heterozygotes exhibit BamC staining in dividing cysts, but also exhibit high background staining (not shown).





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