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Fig. 6. cycA, cycB and cycE messages have longer poly(A) tails in twin mutants. In all panels, + and - refer to the presence or absence of reverse transcriptase (RT) in the reaction. Left brackets show sizes of most abundant poly(A) tails in twin^S1/Df ovaries. Right brackets show sizes of most abundant poly(A) tails in twin/+ ovaries. (A) cycA mRNA. Two different amounts of wild-type (twin/+) RNA are loaded to show the dramatically different distributions of poly(A) tail length in twin mutant versus wild type. The predominant class of poly(A) tail lengths in twin^S1/Df is from 40-80 bp long. The predominant class of poly(A) tail lengths in twin/+ is from 0-20 bp long. (B) The distribution of lengths of cycB poly(A) tails is similar in twin^S1/Df and twin/+. The poly(A) tails may be slightly higher in the twin homozygotes. (C) cycE poly(A) tails tend to be significantly longer in twin^S1/Df than in twin/+. (D) The distribution of bam poly(A) tail length is similar in twin mutant versus wild type. Short tails are more abundant in both genotypes and poly(A) tails reach the same maximum length, although there may be more of the very shortest tails in the wild type. A slow-migrating band is more abundant in twin^S1/Df than in twin/+, but as the band is isolated far above the distribution of poly(A) tails and as BamC levels are lower in twin^S1/Df, this is probably not a reflection of deadenylation defects.

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