|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Ectopically positioned motoneurons at r5 level in reeler mutants.
(A,B) Vibratome sections after wholemount in situ hybridization with an islet
1 riboprobe at E13.5. Level of the vibratome section is indicated on the
whole-mount-stained hindbrain to the right. (A) At r5 level, a compact group
of cells is clearly detectable at the pial surface in control embryos (arrow,
FVM). (B) In reeler mutants, a strong islet 1 labeling is observed at
the ventricular side of the hindbrain (arrow) and a more diffuse group of
islet 1-positive cells is localized in a more medial position (arrowhead).
Abducens nucleus (ab) is labeled near the ventricular zone. Note the absence
of islet 1-positive cells at the pial surface in reeler mutants
(circle). (C-E) Vibratome sections after whole-mount in situ hybridization
with a Ret riboprobe on control and reeler embryos at E12.5
indicates that ectopic r5 MNs retained FVM markers. (C) Ret is expressed at
the end of FVM migration in controls (arrowhead) and in the abducens nuclei
near the floor plate (arrow). (D,E) In reeler hindbrains, ectopically
positioned Ret-positive cells are observed at levels r4/r5 near the
ventricular zone and at intermediate levels (dashed circles), but are absent
at the pial surface (circle). v, ventricular surface; p, pial surface. (F,G)
Cryostat sections at r5 level of E13.5 embryos labeled with an islet 1
riboprobe. (G) Islet 1 labeling is comparable to results obtained from
vibratome sections in reeler mutants: a lack of pial surface labeling
(stipple circle), and ectopic groups of cells within the neural tube and close
to the ventricular side.
|
