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Fig. 1. Physical map of the chn locus and notum bristle phenotypes of the
overexpression of chn and of its interaction with ac/sc. (A)
The structure of the chn and bda transcripts, as deduced by
sequence comparisons of cDNAs and the genomic DNA, are shown. Light rectangles
indicate putative coding regions. The 5' end of chn cDNA is
taken as the origin of coordinates and corresponds to position 231192 of
scaffold AE003812 of the D. melanogaster genome sequence (release
3.1). Available cDNAs suggest a common 5' region in chn and
bda transcripts (red). The possibility of a small intron (hatched
region) in bda has not been ruled out. P elements (open triangles)
are inserted at positions -230 (EPIL6) and -152 (42/18).
Mutation chnECJ1 is associated with a deletion (open
rectangle with uncertainty lines for its end points). The structure of the Chn
protein with the position of the C2H2 Zn-finger motifs (red rectangles) is
indicated. The sequence of three of these motifs is compared with the similar
region of the sequence of the human putative Zf462 protein. (B) Notum of a
wild-type fly. (C) Expression of the EP line EPIL6 with the
MS1096-Gal4 driver (25°C) generates extra bristles. (D) A similar
phenotype is observed by overexpressing UAS-chn with the
C765-Gal4 driver at 18°C. At 25°C most individuals die before
the pharate stage (see below). (E) Halving the genetic dose of ac/sc
largely reduces the effect of overexpressing chn (C765-Gal4
driver, 18°C), and removing ac/sc renders overexpression of
chn inactive in bristle formation (F). (G-I) UAS-sc and
UAS-chn interacted synergistically in the formation of extra
bristles. Flies were cultured at 18°C except that, approximately from 48
to 0 hours before puparium formation, they were kept at 25°C. (G)
UAS-sc; C765-Gal4. (H) UAS-chn; C765-Gal4. (I)
UAS-sc/UAS-chn; C765-Gal4. Note in I, the large increase in macro-
and mesochaetae on the anterior region of the notum (arrowheads).
