|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. (A) Glands inhibited with rFGFR2b were rescued with FGF7 and FGF10, which
increased the number of end buds. Glands were treated with 1.6 µg/ml of
rFGFR2b, and increasing doses of FGFs were added. Glands cultured with rFGFR2b
were rescued only by FGF7 (500 ng/ml) and FGF10 (1000 ng/ml), and not by FGF1
(500 ng/ml) and FGF3 (1000 ng/ml), by other ligands for FGFR2b, or by FGF2
(200 ng/ml) or BMP7 (200 ng/ml). (B) A combination of neutralizing antibodies
to FGF1, FGF7 and FGF10 was required to significantly inhibit branching
morphogenesis (ANOVA, **P<0.01). Individual antibodies
to FGFs (25 µg/ml), or combinations of either two or three antibodies or
similar concentrations of either mouse or goat IgGs, were incubated with E12
SMGs for 48 hours. Individual anti-FGF antibodies or combinations of two
anti-FGF antibodies (not shown) did not significantly inhibit branching. The
number of buds was expressed as a ratio of the number of buds at 48
hours/number of buds at 2 hours (T48/T2).
|
