(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.
Fig. 3. The CLAVATA1 protein and phylogeny. (A) Schematic of predicted domains in
the CLV1 protein. The 21 LRR-motifs are indicated in gray, the kinase domain
is depicted in orange; SP: signal peptide; TM: transmembrane domain. Black
arrows indicate the position of the degenerate primers. (B) Phylogenetic
analysis with LRR-RLK sequences obtained from NCBI or DDBJ databases. The
kinase domain sequences were aligned using the CLUSTALW program
(http://www.ebi.ac.uk/clustalw/-index.html).
The phylogenetic reconstruction was performed with the Minimum
Evolution algorithm provided by the MEGA2.1 software
(Kumar et al., 2001). The tree
indicates the existence of two closely related CLV1-like subfamilies,
including the newly isolated maize sequences ZmKin1, 2, 3 and 5.
Arabidopsis thaliana: CLV1 gi:15222877, RLK1 gi:30698151, RLK2
gi:15229189, RLK3 gi:15235366, RLK4 gi:15225805, RLK5 gi:15220056; Oryza
sativa: OsLRK1 gi:8132685, FON1 AB182388, RLK2 gi:34915202 Glycine
max: GmRLK1 gi:9651941, GmRLK2 gi:9651943, GmRLK3 gi:9651945, GmClv1a
gi:11260266, GmNARK gi:25732530; Pisum sativum: RLK gi:24940244;
Lotus japonicus: RLK gi:24940156; Streptocarpus duneii: RLK
AY061836.
