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Fig. 1. lrdr1 is expressed in dorsal forerunner cells (DFCs) and in
ciliated Kupffer's vesicle (KV). (A) lrdr1 is expressed exclusively
in DFCs (arrow) at 90% epiboly. Dorsal view, anterior towards the top. (B)
Lateral view of an embryo (dorsal on the right) at 80% epiboly stained for
lrdr1 (purple) and lft1 (red) expression. lrdr1 is
restricted to DFCs, whereas lft1 is expressed in margin cells and
midline cells but not DFCs. Sectioned embryos confirmed that lrdr1
(C) is expressed in only DFCs and that lft1 (D) is expressed in cells
adjacent to DFCs at 90% epiboly. No expression of lrdr1 or
lft1 was detected in the yolk (y). (E-G) lrdr1 RNA
expression in KV (arrow) at (E) 6 SS, (F) 8 SS and (G) 11 SS. lrdr1
was also detected in the tailbud at 8 SS (F) and in the floor plate, notochord
and intermediate mesoderm (arrowheads) by 11 SS (G). Posterior-dorsal views
with the anterior to the left. (H-J) Immunofluorescence at (H) 4 SS, (I) 10 SS
and (J) 18 SS using anti-acetylated Tubulin antibodies (green) to detect cilia
and anti-Ntl antibodies (red) to label nuclei in the notochord and KV. Arrows
indicate cilia in KV. Embryos were transversely bisected for visualization of
the tailbud.
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