spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. gbx1 and otx2 expression in Wnt8 gain-of-function experiments. (A) Control embryo stained for gbx1 at 60%. (B-D) Injected embryos with wnt8 RNA 1-5 pg (B), 10-50 pg (C) and 200-400pg (D) stained for gbx1. Overexpression of wnt8 induces ectopic gbx1 expression throughout the epiblast in a dose-dependant way. Overexpression of wnt8 (100 pg) does not induce ectopic gbx1 in Su5402-treated embryos (E) but does so in MZoep embryos (F). (G) Control embryo stained for otx2 at 60%. (H-J) Injected embryos with wnt8 RNA 1-5 pg (H), 10-50 pg (I) and 200-400 pg (J) stained for otx2. Overexpression of wnt8 inhibits otx2 expression. Overexpression of wnt8 (100 pg) in Su5402-treated embryos (K) and in MZoep embryos (L) inhibits otx2 expression. (M-O) wnt8 expression in wild type at 60% (M), in Su5402-treated embryos (N) and in MZoep embryos (O). (A-L) Animal pole views. (M-O) Dorsal views, anterior upwards.





Right arrow Return to article