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Fig. 5. gbx1 and otx2 expression in Wnt8 loss-of-function
experiments. (A-C) Embryos stained for gbx1 and (D-F) embryos stained
for gbx1 and Ntl protein (brown). (A-C) Control embryo (A), embryo
injected with MoWnt8 (B) and Df(Wnt8) mutant embryos (C) at 60%.
gbx1 is not expressed at 60% in absence of Wnt8. (D-F) Control embryo
(D), embryo injected with MoWnt8 (E) and Df(Wnt8) mutant embryo (F)
at 80%. gbx1 expression is observed at 80% onwards and overlaps
partially with the Ntl domain. (G-L) Embryos stained for otx2 and Ntl
protein (brown). (G-I) Control embryos (G), embryo injected with MoWnt8 (H)
and Df(Wnt8) mutant embryo (I) at 60%. (J-L) Control embryos (J),
embryos injected with MoWnt8 (K) and Df(Wnt8) mutant embryo (L) at
80%. Laterally a posterior shift of the otx2 expression domain is
visible (red arrowheads) in the morphants and in the Df(Wnt8) mutant
embryos. (A-L) Dorsal views, anterior upwards.
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