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Fig. 6. Ubiquitous misexpression of Slit2 causes posterior pathfinding errors of
OSN axons. (A) Schematic of the experimental procedure. (B) A heterozygous
hsp:slit2-gfp transgenic embryo overexpressing a Slit2-GFP fusion protein
(green) and wild-type siblings at 24 hpf, which had received heat-shock
treatment at 19 hpf. (C-H) One wild-type and two hsp:slit2-gfp/+
examples are shown. In Slit2-overexpressing embryos, some axonal fibers are
deviated from the normal pathway and extend posteriorly (arrowheads and arrows
in E-H), occasionally crossing the midline (arrows in E,F). C,E,G, frontal
views with dorsal to the top; D,F,H, dorsal views with anterior to the left.
Scale bar: 100 µm.
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