DEV01693 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. caNotch⁺ cells are positive for GFAP in layers adjacent to the lateral ventricle at P15, after transfection of pCAG-ExNotchZ at E13.5. Green and red signals show EYFP fluorescence (A,C) and immunostaining with the anti-GFAP-antibody (B,C), respectively. All three panels show the same section. C is a merged view. Scale bar: 100 mm.

• Supplemental Figure 2 -

  Fig. S2. P5 cortex after cotransfection of pCAG-ExZ and pXCANCre at E13.5. Green and blue signals show EYFP fluorescence (A) and immunostaining with the anti-b-gal antibody (B), respectively. Patterns of EYFP⁺/b-gal⁺ neuron distribution were the same as those observed following transfection of pCAG-ExZ alone (see Fig. 2A,B). Scale bar: 200 mm.

• Supplemental Figure 3 -

  Fig. S3. P5 cortex after cotransfection of pCAG-ExNotchZ and pXCANCre at E13.5. Green and blue signals show EYFP fluorescence (A) and immunostaining with the anti-b-gal antibody (B), respectively. Populations of EYFP⁺/b-gal⁺ neurons shifted upward slightly compared with those transfected with pCAG-ExZ alone (see Fig. 2A,B). Scale bar: 200 mm.

• Supplemental Figure 4 -

  Fig. S4. The removal of caNotch at E17.5 results in a further shift of generated neurons to an upper part of layers II and III. P5 cortices after electroporation of pCAG-ExZ at E17.5 (A), or double electroporation of pCAG-ExNotchZ at E13.5 and pXCANCre at E17.5 (B,C), are shown. Green and blue signals show EYFP fluorescence (A,B) and immunostaining with the anti-b-gal antibody (C), respectively. The same section is shown in B and C. Scale bar: 200 mm for A-C.

• Supplemental Figure 5 -

  Fig. S5. Upper-layer neurons generated from transplanted progenitors are positive for Lhx2. A magnified view of an area of layers II and III shown in Fig. 9C,D is shown. Blue and red signals show immunostaining with the antibodies recognizing b-gal (A,C) and Lhx2 (B,C), respectively. The same section is shown all panels; C is a merged view. Scale bar: 20 mm.

• Supplemental Figure 6 -

  Fig. S6. Neurons that are generated from transplanted progenitors in the host brain occupy layers II and III. P5 cortex after transplantation of progenitors at E13.5 and BrdU labeling at E14.5 is shown. Progenitors were prepared after double electroporation of pCAG-ExNotchZ at E13.5 and pXCANCre at E15.5. Blue and red signals show immunostaining with the anti-b-gal (A,C) and anti-BrdU (B,C) antibodies, respectively. The same section is shown in all panels; C is a merged view. Scale bar: 20 mm.