|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Neurogenesis resumes after the removal of caNotch. P5 brains after
single electroporation of pCAG-ExNotchZ at E13.5 (A,E-G), and after double
electroporation of pCAG-ExNotchZ at E13.5 and pXCANCre at E15.5 (B-D,H-K).
(E-G) Magnified view of an EYFP+ region close to the lateral
ventricle (LV). (H-K) Magnified views of layers II and III. Green and blue
signals show EYFP fluorescence (A,B,E,G,H), and immunostaining with the
anti-ß-gal antibody (C,D,I,K), respectively. Red signals show
immunostaining with antibodies recognizing FLAG (F-H) and Lhx2 (D,J,K). B-D,
E-G, and I-K each show the same section; D,G,H and K are merged views. Note,
EYFP+ neurons only signal green, no yellow colouration was observed
even after the merge of two colors (H) because of a lack of FLAG signals. All
ß-gal+ neurons of layers II and III were positive for Lhx2.
80.5±2.0% of ß-gal+/Lhx2+ neurons expressed
Lhx2 at high levels. The ratio was almost identical to that of strongly
Lhx2-positive cells in the total Lhx2+ population of layers II and
III (79.6±2.45%). A similar distribution of cells was also confirmed in
five brains by using EYFP fluorescence and X-gal staining (data not shown).
Scale bars: 200 µm for A-D; 20 µm for E-H,I-K.
|
