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Fig. 7. Neurons of layers II and III are not born from caNotch+ cells
before Cre transfection. P5 cortices after electroporation of
pCAG-EYFP (A-D) or pCAG-ExNotchZ (E-H) at E13.5, BrdU labeling at E14.5 and
electroporation of pXCANCre at E15.5, are shown. Red and green signals show
immunostaining with the anti-BrdU and anti-GFP antibodies, respectively.
Arrowheads and arrows indicate neurons that were heavily and lightly labeled
with BrdU, respectively. C and G are merged views of the sections shown in A
and B, and E and F, respectively. (D,H) Ratios of neurons that were heavily
(h) and lightly (l) labeled with BrdU in the EYFP+ population. Each
ratio was calculated from five brains. After EYFP transfection,
layers II and III contained 17.4±1.6% heavily- and 49.8±2.9%
lightly-labeled neurons, whereas layer IV contained 68.8±6.6% heavily-
and 21.2±5.8% lightly-labeled neurons. EYFP/caNotch
transfection showed 0.7±1.1% heavily and 14.0±1.3%
lightly-labeled neurons in layers II and III. After
EYFP/caNotch transfection, many EYFP+ cells were
not labeled with BrdU in the upper layers (85.8±1.8%), presumably
because the amount of BrdU in nuclei was reduced to below the detection limit
after multiple cell divisions. Scale bars: 200 µm for A,E; 20 µm for
B,C,F,G.
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