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Fig. 8. EYFP+/ß-gal+ neurons of layers II and III are
born after Cre transfection. P5 cortices after double electroporation
of pCAG-EYFP (A-D) or pCAG-ExNotchZ at E13.5 (E-H), and of pXCANCre at E15.5,
and after BrdU labeling at E16.5, are shown. Red and green signals show
immunostaining with the anti-BrdU and anti-GFP antibodies, respectively.
Arrowheads indicate neurons that were heavily labeled with BrdU. C and G are
merged views of the sections shown in A and B, and E and F, respectively.
(D,H) Ratios of neurons that were heavily (h) and lightly (l) labeled with
BrdU in the EYFP+ population. Ratios were calculated from 5 brains.
After EYFP transfection, layers II and III contained 46.1±3.7%
heavily- and 12.0±3.4% lightly-labeled neurons, whereas layer IV
contained 2.9±0.1% heavily- and 1.3±1.1% lightly-labeled
neurons. EYFP/caNotch transfection showed 48.1±3.5%
heavily- and 8.2±2.3% lightly-labeled neurons in layers II and III.
Scale bars: 200 µm for A,E; 20 µm for B,C,F,G.
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