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Fig. 9. caNotch+ progenitors lose the ability to generate low-layer
neurons. Shown are P5 cortices after transplantation at E13.5 of progenitors
that had been prepared after electroporation of pCAG-ExZ at E13.5 (A,E), after
double electroporation of pCAG-ExZ at E13.5 and pXCANCre at E15.5 (B,F), or
after double electroporation of pCAG-ExNotchZ at E13.5 and pXCANCre at E15.5
(C,D,G). Blue and red signals show immunostaining with antibodies recognizing
ß-gal (A-D) and Lhx2 (D), respectively. C and D show the same section. D
is a merged view. All ß-gal+ neurons were also positive for
Lhx2. E15.5 progenitors single transfected with pCAG-ExZ at E13.5 showed the
same results as did E15.5 progenitors double transfected with pCAG-ExZ at
E13.5 and pXCANCre at E15.5 (data not shown), confirming that Cre did not
affect the laminar fate of neurons. (E-G) Ratios of ß-gal+
neurons that were distributed to each layer of the cortex. Each ratio was
calculated from 10 brains. Results were as follows. In E: layers II and III,
23.8±9.1%; layer IV, 32.7±5.1%; layer V, 40.1±8.7%; layer
VI, 0.3±0.5%; and white matter (WM), 3.1±2.3%. In F: layers II
and III, 97.1±3.5%; layer IV, 1.0±1.2%; layer V,
0.9±0.7%; and WM, 1.1±3.1%. In G: layers II and III,
89.7±7.4%; Layer IV, 4.3±3.5%; layer V, 2.0±4.2%; layer
V1, 1.8±0.5%; and WM, 0.3±1.1%. Scale bar: 150 µm for
A-D.
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