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Fig. 3. HE-staining of horizontal tissue sections through the olfactory epithelia and olfactory bulbs of mouse embryos at E12.5 and E18.5. The sections were cut from wild-type embryos (Wt1+/+) and from age-matched mice with specific lack either of the Wt1(-KTS) or the Wt1(+KTS) splice variant. Strikingly, the Wt1(+KTS)-deficient embryos (I-K) exhibited thinner olfactory epithelia than wild-type animals (A-C) and embryos with lack of the Wt1(-KTS) protein (E-G). (D,H,L) The olfactory bulb in the Wt1(+KTS)-deficient (L) embryo is reduced in size and hypocellular compared with the wild-type embryos (D). Olfactory bulb morphology appeared normal in embryos with inactivation of Wt1(-KTS) (H). Representative data for five embryos from each group are shown. Scale bars: 100 µm.





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