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Fig. 6. Mash1 mRNA (A) and protein (B) in human embryonic kidney (HEK) 293 cells,
which had been stably transfected either with empty expression vector (HEK293)
or with the Wt1(-KTS) and Wt1(+KTS) splice
variants, respectively. (A) Mash1, GAPDH and Wt1 transcripts
were detected by reverse transcription PCR. Data shown are representative for
the three independent clones that were analysed. Stable expression of
Wt1(+KTS), but not of the -KTS variant, induced Mash1 mRNA in HEK293
cells. (B) Stimulation of Mash1 by the Wt1(+KTS) product was
confirmed by immunoblotting with a polyclonal anti-Mash1 antibody. (C) A
partially overlapping pattern of Wt1 (green) and Mash1 (red) was revealed by
double-immunostaining in cells of the developing olfactory epithelium (E18.5).
Scale bars: 100 µm in C, parts a, b, c; 10 µm in C, part d.
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