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Fig. 1. Cited2 is differentially expressed in the heart and elsewhere
during development. (A) Anterior view of a late headfold stage embryo (7.5
dpc) showing expression in the ventral node (arrow), cardiac crescent and
blood islands. (B) Ventral view of a flatmounted four-somite stage embryo (8
dpc) showing expression in the cardiac crescent and in the caudal lip and pit
of the node (arrow). (C) Ventral view of a flatmounted six-somite stage embryo
(8 dpc) showing expression in the cardiac crescent, anterior lateral mesoderm
(black arrow), paraxial mesoderm (white arrow) and node. (D) Anterior view of
a six-somite stage embryo showing elevated expression in the cardiac crescent,
anterior lateral mesoderm and trunk paraxial mesoderm. (E,F) Transverse
sections through D at the levels indicated showing elevated expression in the
cardiac mesoderm (arrow) and low-level expression throughout. (G) Lateral and
dorsal view of 21-somite stage embryo (9.5 dpc) showing elevated expression in
the forebrain-midbrain boundary, branchial arches 1 and 2, heart, and somites.
(H) Dorsal view of the embryo in G showing expression in the hindbrain
(rhombomeres 3, 5, 6, 7) and adjacent paraxial mesoderm. (I-K) Frontal
sections through a 21-somite stage embryo at the levels indicated in G. (I)
Expression is elevated in the ventricular myocardium and trabeculae of the
outflow tract and presumptive right ventricle; (J) in the myocardium and, to a
lesser extent, the endocardium and endocardial cushion cells in the outflow
tract and atrioventricular canal; and (K) in the aortic sac, presumptive atria
and sinus venosus (inflow tract). (L) Lateral view of 40-somite stage embryo
(10.5 dpc) showing expression in the outflow tract, atrioventricular canal
(black arrow), branchial arch 4 (white arrow), central nervous system, somites
and limbs. (M,N) Sections through a 40-somite stage embryo at the levels
indicated in L, showing: expression in the (M) outflow tract and, to a lesser
extent, in the compact myocardium and trabeculae of the ventricles; and
expression in the (N) myocardium adjacent to the endocardial cushion of the
atrioventricular canal. (O) Ventral and (P) dorsal views of a heart dissected
from an embryo at 13.5 dpc showing expression predominantly in the outflow
tract (O) and inflow region (P). (Q) Frontal section through a dissected heart
at the same stage as shown in O,P showing expression in the septum primum
(black arrow), around the vena cava (asterisk) and in the endocardial cushions
of the atrioventricular canal (white arrow). Cited2 expression was
assayed by detecting ß-galactosidase activity from the
Cited2-lacZ allele in embryos heterozygous (A,B,G,H,L,O,P)
or homozygous (I,J,K,M,N,Q) for the allele, or via Cited2 RNA
hybridization (C-F). The colorimetric reaction used to identify
Cited2 transcripts was extended for the embryo in D, in order to
reveal low levels of Cited2 expression. cc, cardiac crescent; oft,
outflow tract; ift, inflow tract; v, ventricle; avc, atrioventricular canal;
as, aortic sac. Scale bar: 135 µm in A,B; 260 µm in C,D,H; 35 µm in
E,F; 280 µm in G; 50 µm in I-K; 550 µm L; 94 µm in M,N; 390 µm
O,P; 310 µm Q.
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