DEV01678 Supplementary Material

Files in this Data Supplement:

• Supplemental Table 1 (Adobe PDF)
• Supplemental Table 2 (Adobe PDF)
• Supplemental Figure 1 -

  Fig. S1. Nmyc immunohistochemistry. Immunostaining of control 293 cells (A) and cells transfected with vector expressing NmycEGFP (B). Note nuclear localization of fusion protein.

• Supplemental Figure 2 -

  Fig. S2. Evidence that cre recombinase is active throughout the epithelium. Sftpc-cre;Nmyc^flox mice were crossed with Rosa26R reporter mice also carrying the Nmyc^flox allele. (A) Control lung showing absence of endogenous lacZ activity. (B) b-Gal staining shows recombination has taken place throughout the epithelium of normal cre(+);Rosa26R(+);Nmyc^+/+ lung at E16.5. (C) Confirmation of recombination throughout the epithelium of cre(+);Rosa26R(+);Nmyc^+/+ lung at E18.5. (D) Evidence that recombination has occurred in the cells lining the abnormal sacs of cre(+);Rosa26R(+);Nmyc^flox/flox E18.5 lung. Scale bars: 50 mm.