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Fig. 7. Conditional deletion of Nmyc results in severely abnormal lung
development. (A) Gross morphology of lungs at E18.5. The lung that is
cre(+);Nmycflox/+ (middle panel) has a mosaic phenotype
with some normal tissue (white box). By contrast, all
cre(+);Nmycflox/flox lungs (right panel) had a much more
uniform abnormal phenotype. (B) Sections of E18.5 lungs after staining with
Hematoxylin and Eosin. In this case, two examples of
cre(+);Nmycflox/+ lungs are shown (middle panels): the one
on the left has a normal morphology and resembles a cre(-) lung (far left),
whereas the one on the right is severely abnormal and resembles a
cre(+);Nmycflox/flox lung (far right), except that regions
of normal tissue are present (black box enlarged in inset). (C,D) Sections of
wild-type and abnormal heterozygous cre(+);Nmycflox/flox
or Nmycflox/+ lungs at E14.5 (C) and E15.5 (D). (E) Level
of Nmyc RNA in conditional mutant lungs as measured by RT-PCR of
total RNA. Note the significant decrease in RNA levels at E14.5-E16.5.
Asterisk indicates abnormal cre(+);Nmycflox/+ lung at
E15.5. (F) Nmyc antibody staining of wild-type and cre(+);
Nmycflox/flox lungs at E18.5. Note there is only weak
staining of cells in which Nmyc has been deleted. Scale bars: in B,
200 µm; in C,D, 100 µm; in F, 50 µm.
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